Journal: BMC Ophthalmology

Article Title: Retinal upregulation of inflammatory and proangiogenic markers in a model of neonatal diabetic rats fed on a high-fat-diet

doi: 10.1186/1471-2415-13-14

Figure Lengend Snippet: TNF-α retinal expression. The immunohistochemical expression of TNF-α was found to be upregulated in vessels of the inner retina of the diabetic group fed on a HFD (arrows) compared to diabetic group on a normal diet and controls. Barr 10 μm. Western blot confirms the higher expression in the diabetic group on a HFD. (***p < 0.0005).

Article Snippet: The following primary antibodies were used for immunohistochemical analyses: The anti-mouse polyclonal antibody against vascular endothelial growth factor (VEGF) (1:500) (Santa Cruz Biotechnology Inc. 2145 Delaware Avenue Santa Cruz, CA, USA); the anti-mouse polyclonal antibody against receptor advanced-glycation end products (RAGE) (1:300) (AnaSpec Inc, San Jose, CA, USA); the anti-mouse polyclonal antibody against 5-lipoxygenase (5-LO) (1:500) (Assay biotechnology company, San Francisco, CA, USA) and the anti-mouse polyclonal antibody against tumor necrosis factor-alpha (TNF-α) (1:100) (Assay Biotechnology Company, San Francisco, CA, USA).

Techniques: Expressing, Immunohistochemical staining, Western Blot