Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Staphylococcal entertotoxins of the enterotoxin gene cluster (egcSEs) induce nitric oxide- and cytokine dependent tumor cell apoptosis in a broad panel of human tumor cells

doi: 10.3389/fcimb.2013.00038

Figure Lengend Snippet: Anti- TNF-α and NO inhibitor, alone and in combination inhibit Hep-2 tumor cells cytotoxicity induced by 10% supernatants from SEA and egcSE-stimulated PBMCs . Hep-2 tumor cells cytotoxicity induced by 10% supernatants from SEA and egcSE-stimulated PBMCs before (black) or after treatment with L-NMMA (dark gray) or anti-TNF-α antibody (light gray) or a combination of L-NMMA plus anti-TNF-α antibody was analyzed by flow cytomerter using FITC-conjugated annexin-V and propidium iodide (IP) staining. The results are expressed as the mean ± SEM ( n = 3 independent experiments). Asterisks indicate statistical significance compared to values obtained before treatment with L-NMMA or anti-TNF-α antibody.

Article Snippet: PBMC supernatants were incubated with and without 30 μg/mL of mouse neutralizing polyclonal anti-TNF-α antibody (Abcys, Paris, France) for 18 h at 22°C before carrying out the cytotoxic assays described above.

Techniques: Staining

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Staphylococcal entertotoxins of the enterotoxin gene cluster (egcSEs) induce nitric oxide- and cytokine dependent tumor cell apoptosis in a broad panel of human tumor cells

doi: 10.3389/fcimb.2013.00038

Figure Lengend Snippet: Level of cytokines in supernatants of PBMC stimulated by SEA, SEG, SEI, SElM, SElN, and SElO .

Article Snippet: PBMC supernatants were incubated with and without 30 μg/mL of mouse neutralizing polyclonal anti-TNF-α antibody (Abcys, Paris, France) for 18 h at 22°C before carrying out the cytotoxic assays described above.

Techniques:

Journal: BMC Ophthalmology

Article Title: Retinal upregulation of inflammatory and proangiogenic markers in a model of neonatal diabetic rats fed on a high-fat-diet

doi: 10.1186/1471-2415-13-14

Figure Lengend Snippet: TNF-α retinal expression. The immunohistochemical expression of TNF-α was found to be upregulated in vessels of the inner retina of the diabetic group fed on a HFD (arrows) compared to diabetic group on a normal diet and controls. Barr 10 μm. Western blot confirms the higher expression in the diabetic group on a HFD. (***p < 0.0005).

Article Snippet: The following primary antibodies were used for immunohistochemical analyses: The anti-mouse polyclonal antibody against vascular endothelial growth factor (VEGF) (1:500) (Santa Cruz Biotechnology Inc. 2145 Delaware Avenue Santa Cruz, CA, USA); the anti-mouse polyclonal antibody against receptor advanced-glycation end products (RAGE) (1:300) (AnaSpec Inc, San Jose, CA, USA); the anti-mouse polyclonal antibody against 5-lipoxygenase (5-LO) (1:500) (Assay biotechnology company, San Francisco, CA, USA) and the anti-mouse polyclonal antibody against tumor necrosis factor-alpha (TNF-α) (1:100) (Assay Biotechnology Company, San Francisco, CA, USA).

Techniques: Expressing, Immunohistochemical staining, Western Blot